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Developed with a high concentration 4x mix format, qPCRBIO Probe 1-Step Virus Detect offers greater sensitivity and flexibility by allowing more sample to be added to each reaction, and for smaller reaction volumes to be used with confidence. This is particularly beneficial in high throughput assays or for clinical diagnostic testing where accurate and reproducible detection of low copy viral templates is of crucial importance. This specialised kit allows accurate and sensitive detection of viral sequences over a broad range of input RNA, down to 4 copies tested per reaction (0.8 copies per ?L). Fast and efficient cDNA synthesis is provided by UltraScript Reverse Transcriptase, which can operate over a wide range of reaction temperatures. Reverse transcription can be performed in only 5 minutes and at temperatures up to 55°C, enhancing detection of GC-rich and highly structured viral RNA sequences. The PCR step is powered by PCRBIO HS Taq DNA Polymerase, which employs antibody-mediated hot start technology for specific amplification of virus-derived cDNA, with improved tolerance to common PCR inhibitors. Combined with a specially formulated buffer system developed using proprietary smart screen technology, qPCRBIO Probe 1-Step Virus Detect gives robust and reproducible RT-qPCR while minimising the potential for primer dimer and other non-specific products. qPCRBIO Probe 1-Step Virus Detect comes ready-optimised and validated for qualitative detection of SARS-CoV-2 nucleic acid and is ideally suited to high throughput testing of COVID-19 clinical samples, either by laboratory developed assays or as a component of diagnostic testing kits. Validation has been performed using the Charité (Berlin, Germany) recommended primer-probe sequences (RdRp and E genes), and Centers for Disease Control and Prevention (Atlanta, USA) primer-probe sequences (N gene).
qPCRBIO Probe 1-Step Virus Detect contains all the required components for accurate and sensitive RT-qPCR except for primers, probe, RNA template and water. For maximum flexibility the kit is engineered for use on a wide range of probe technologies, including TaqMan?, Scorpions? and molecular beacon probes. It can be used with RNA extracted by most commercial kits, provided the amount and quality of template RNA are within an acceptable range.?The kit is compatible with multiplexing assays and with all real-time PCR platforms.?Use our?qPCR Selection Tool?to find out which ROX variant is compatible with your instrument.